Roche PCR Amplification Product Selection Guide

FastStart Series Taq DNA Polymerase - a high performance hot start enzyme

The active site of FastStart Taq DNA Polymerase is modified by a chemical group to completely block the amplification activity below 70Â°. The performance is extremely stable. The FastStart High Fidelity PCR System has 6X fidelity and can be used to amplify fragment lengths up to 5kb.

Expand High Fidelity PCR System - Experience strong enzyme activity,
The most applicable amplification scheme

The main component of the reagent is a high-purity Taq enzyme and a protein combination with corrective activity to obtain high yield and high fidelity amplification products in a wide range of applications.

Especially recommended for difficult to amplify templates and low concentration templates;
High enzyme activity and high fidelity amplification are ideal for DNA fragment cloning, labeling and other applications.

Â Â Â GC-RICH PCR System - specifically for high GC and repeat sequence templates

DNA polymerase and PCR product selection guide:

		Hot Start			Special amplification application
PCR application	Conventional PCR	Rapid PCR	Hot Start	Multiplex PCR/sequencing	widely used	Ultra high fidelity	Difficult template	Long fragment
Recommended Products	Taq DNA Polymerase	AptaTaq FastStart DNA Polymerase	FastStart Taq DNA Polymerase	FastStart High Fidelity PCR System	Expand High Fidelity PCR System	Pwo SuperYield DNA Polymerase	GC-RICH PCR System	Expand Long Range
characteristic	consistency	fast	Specificity	accuracy	Strong enzyme activity	High fidelity	reliability	stability
Maximum amplified fragment length	Up to 3 kb	Up to 500 bp	Up to 3 kb	Up to 5 kb	Up to 5 kb	Up to 3 kb	Up to 5 kb	5 to 25 kb**
Specificity	**	****	****	****	***	**	***	***
Sensitivity	**	**	****	****	***	**	***	**
Continuous synthesis ability	**	****	***	***	****	**	***	***
Fidelity (vs.Taq)	1x	1x	1x	6x	3x	18x	3x	3x
Prevent cross-contamination	Yes	Yes	Yes	Yes	No*	No	No	No
Dosage (Units/50ul)	1.25	2/20ul	2	2.5	2.6	2.5	2	3.5
Molecular Cloning	TA cloning	TA cloning	TA cloning	TA cloning	TA cloning	Blunt end cloning	TA cloning preferred over Blunt end cloning	TA cloning
Single enzyme	âˆš	âˆš	âˆš	âˆš	âˆš	âˆš	âˆš
Single enzyme, GMP level	âˆš
Enzyme + dNTPs packaging	âˆš		âˆš	âˆš	âˆš	âˆš	âˆš	âˆš
Premix	âˆš	âˆš	âˆš		âˆš	âˆš

Note:
*To prevent cross-contamination performance: please select Expand High FidelityPLUS PCR System
**Longer fragment amplification (20-35kb): Please select Expand 20 kbPLUS PCR System

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