The eyepiece and objective of the microscope are usually not installed before they are used. Therefore, the eyepiece and objective should be installed before each use of the microscope. In fact, the installation of the microscope eyepiece is not difficult to operate, but the objective lens for installing the microscope may be tricky for beginners. Note that the objective lens should be installed correctly. At the same time, because the objective lens is usually expensive, and in order to ensure better safety, it is recommended to use the left index finger and the middle finger to support the microscope objective lens, and then carefully install the objective lens with the right hand, so that even once The installation is good, and the objective lens should be prevented from falling to the ground and damaged.
At the same time, light operation is also a very important step when using a microscope. First of all, it should be noted that the light is not free to turn an objective lens to the light-passing hole, but must first use the low-power lens to perform the light, and when the light is strong, apply a small aperture, a plane mirror, and the light is weak. When using a large aperture, a concave mirror, and the mirror should also be rotated with both hands until a uniform bright circular field of view is observed. And after the completion of the light, it is not appropriate to move the microscope at random to avoid the light from entering the light-passing hole through the mirror accurately, thus affecting the observation.
When using a quasi-focus spiral to adjust the focal length, accurately finding the object is also an important step in the use of the microscope. Remember not to have the following error in the operation:
1. Direct focus adjustment under high power microscope;
2. Regardless of the rise or fall of the lens barrel, the eye always observes the field of view in the eyepiece;
3. I don't know the critical value of the object distance. When the object distance is adjusted to 2cm-3cm, it is still up, and the speed of rotating the quasi-focus spiral is too fast.
It should be noted that the first two errors often cause the objective lens to touch the film and damage the film or lens, and the third error is a common mistake that is often used when the beginner uses the microscope. When adjusting the focal length, be sure to adjust it under the low magnification lens. First, turn the coarse focus screw to make the lens barrel slowly descend, so that the objective lens slowly approaches the slide, but at the same time, be careful not to let the objective lens touch the glass. In this process, pay attention to the eye should look at the objective lens from the side, then use the left eye to look into the eyepiece, and slowly adjust the coarse focus spiral spiral, while slowly raising the lens barrel until the object is observed Like so far. At the same time, it should be understood that usually the object distance of the microscope is kept at about 1cm, so if the object distance has been far more than 1cm, and the object image is still not observed, it may be because the specimen is not in the field of view or at the rotation of the coarse quasi-coil spiral speed. Fast, then you should adjust the loading position first, then repeat the above steps, and when there is a blurred object image in the field of view, you should use a fine focus screw to adjust, to narrow the search range and improve the search. The speed of the object.
At the same time, when using the low power mirror and replacing the high power mirror, do not directly rotate the objective lens with your fingers. This may cause the optical axis of the objective lens to be deflected. Because the material of the converter is soft and the precision is high, the thread is not stressed. Evenly, it is easy to loosen. And once the thread is damaged, the entire converter may eventually be scrapped. Therefore, during operation, the lower rotating plate of the converter should be held to convert the objective lens.
At the same time, when observing an object with an optical microscope, both eyes should be opened at the same time, and then the left eye should be used to look into the eyepiece. Be careful not to close your right eye. This is not in line with the experimental observation requirements, and this bad habit can also cause fatigue in the left eye. Secondly, it is impossible to draw while watching.
It is recommended to do this practice several times before the actual use: first open your eyes, use a piece of paper or your own palm to stand between the two eyes, that is, the nose and the front, and the left and right eyes can not see each other. Then look at the left side of the eye, then look at the right side, so 3-5 times, do it every morning and evening, usually around a week.
The above points are common problems in the use of microscopes. In fact, it is not difficult to correct and overcome these problems. It is only necessary for us to practice more and use the microscope better.
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