1. Materials and Methods 1 The frog pond is constructed with a frog pond of 20m5m and a water depth of 0.6m to 0.8m. A green island of 1.5m1.5m is set in the pool. The hatch pool and Dianchi Lake built a 20m4m earthen pond. Each pool is easy to drain into and out of the basin. The height of the surrounding walls of the pool is 1m. The perimeter of a 2m wide soil raft is 1.2m high. 2 Collection and holding of frogs In March 1999, 80 frogs were purchased from Jiangsu, Zhejiang, and Hubei, and more than 400 grams were collected. There were 40 females with an average of 410 g and 40 male frogs with an average of 425 g. After the frogs are collected, they are put into a frog pond for raising. There are 8 food counters in the pool, 4 in the water and 4 on the island. Another assembly of two 40W black lights. The pool is always running and the water is fresh. Rich in dissolved oxygen, pH value of 7.0 ~ 7.5, water depth of 0.3 ~ 0.6m, inlet and outlet are equipped with rodents, snakes, frogs, fish and other enemy organisms into the net. Prior to stocking the frogs, the aquaculture pond was disinfected with quicklime. Quantitative feeding of live bait, small fish, shrimp and other live bait is performed on a regular basis. In the evening, black light traps are activated to intensify cultivation and promote gonad development. 3 frog selection and matching ursin frogs choose healthy and lively individuals with intact and undamaged body surface. Female frogs are swollen in the abdomen, soft and elastic; male frog throat golden skin, forelimb stout, obvious marriage tumors. Artificial oxytocin 20 groups, natural breeding 20 groups, male to female ratio is 1:1. 4 The oxytocin-releasing hormone analogue LRH-A and human chorionic gonadotropin HCG were used as oxytocic oxytocicants and formulated with 0.7% physiological saline. Oxygen-producing apparatus is disinfected at high temperatures. Female frogs are injected with LRH-A200ug and HCG2000 international unit mixed liquid per kilogram. Each injection is 1 to 1.5 mL, and the male frog dose is reduced by half. Both are intramuscular injections of the buttocks. 5 Spawning and hatching The spawning pond is divided into two parts by a net, one part is placed in a frog without hormone injection, and the other part is placed in a hormone-injected frog for natural reproduction. After spawning, the egg masses are transferred to the hatching pool after hatching. Second, the test results 1 frog's gonadal development According to the preliminary observational study, from the start to the frog sexual maturity is initially 1 foot age, weight 250g/above, the female frogs of different body weight were anatomically observed in Table 1, gonadal development and maturation In the female frog, the ovary almost filled the entire abdominal cavity, and the fat body was large. The ovary weight, absolute fecundity, and sexual maturation coefficient of the female frog all increased with the body weight. 2 Oestrus and Spawning After frogs were given hormone injections at 17 o'clock on the 8th of May, they began to reduce their activities and their vocalizations decreased. They began spawning the next morning and ended before 10 a.m. On the next morning, spawning occurred one after another. The hormone effect time was 40 hours. The artificial frog-feeding frogs 20 groups were all produced, and the rate of production was 100%. In the frog pond, there are eggs on the top of the peanut and on the bank slope, and some eggs are not picked up and sank into the water. From the results of oxytocin production, artificial spawning groups spawned at short intervals and spawning periods were relatively uniform. The average spawning rate was 6 669 per pod, and the average fertilization rate was 90%. Natural breeding pro-frogs laid eggs one after another on May 18, with a longer interval until July 31. They spawned 11 eggs, and the average egg-laying capacity was 4838 eggs/day, the spawning rate was 55%, and fertilization rate. This is 83%, which is lower than the artificial urine production group. 3 Incubation The egg masses obtained by artificial oxytocin were transferred to hatching pond for incubation. The hatching water temperature was 19 to 27°C. After 10 days, the fish hatched out of the film and counted after eating. Each pod had a total of 4680 tails, and the average hatching rate was 80%. . In the natural breeding group, the hatching time was also inconsistent due to the inconsistent hatching time. The average hatched larvae were hatched with 1935 larvae and the hatching rate was 40%. During the incubation process, check the egg blocks and find that the dead eggs are removed in time. Occurrence of egg mold must be controlled in time. Remove predators in the pool. Check the water inlet and drain system frequently to eliminate the dirt on the screens. It was found that the breakage of the screens should be replaced to prevent large scales such as sword leeches from invading the fertilized eggs. 4 Seedling cultivation At the same time as the hatching of the eggs, the algae is started to be cultured with water, and 7 to 10 days after the algae content in the water reaches 100,000 per ml, it can be used as an open bait. The newly hatched cockroach has a body length of 5 to 6.3 mm, a body width of 4 mm, and a weak constitution. When the water temperature is 20 to 25°C, the communication time for the digestive tract is 6 to 7 days. For any diet, nutrition is mainly provided by the yolk of its abdomen. On the 8th day, fully cooked egg yolks were fed, and the yolks were wrapped with gauze or Nylon sieves during the feeding process. The yolks were kneaded in a container and rinsed to form fine suspended egg yolks. The spoons were evenly sprinkled in the pool. Feed each at 8am and 16pm each day. After 15 days, feed the amount of soymilk, bean curd residue, flour, wheat bran, or cooked squid, etc., and feed them 3% to 5% of the total weight of the quail. 30-day-old clams should be stocked in graded pools. Usually, there should be 2 to 4 bait stations per pond, with an area of ​​about 0.5m2, and about 20cm below the water surface. The artificial bait is soaked and put on it after feeding. The daily feeding amount is 5% to 8% of the body weight, and the bait is the best fish meal. It can also be fed with granular food and fed twice a day. The water-soluble oxygen is kept above 3.5 mg/L, pH 6.5-7.5, the transparency of water is 25-30 cm, and the optimum water temperature is 23-25°C. Summer shade, pay attention to regulate temperature, change the water every 3 days. About 70 days after hatching, the forelegs reach out and the tail absorbs. At this time, they do not eat bait and they cannot dive for a long time. The respiration is also carried out by the lungs due to degeneration. Therefore, planks and other floating objects should be placed on the lungs for landing. . When more than 90% of the fish become frogs, they can be picked up and transferred to the young frog pool. After metamorphosis frogs average weight 6g, body length 4cm. The entire process was 85 days from the time the eggs were released to the abnormal frogs. China Agricultural Network Editor
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