Students who suffer from multiple genes or LncRNA knockouts, your gospel is coming!
We all know that the genetic scissors CRISPR/CAS9 technology is a big fire this year. The reason why this technology is so hot and its convenience, efficiency, accuracy and high cost performance for genetic manipulation are inseparable. CRISPR/Cas9 is a technology that enables precise editing of specific sites in the genome of any species. The principle is that the endonuclease Cas9 protein recognizes a specific genomic locus and cleaves double-stranded DNA through a guide RNA (gRNA). Once the DNA strand is cleaved, it is possible to introduce knockouts, knock-ins, mutations, etc. for specific genes.
Legend: Gene knockout, knock-in and mutation based on non-homologous end-repair and homologous recombination
However, the CRISPR-CAS9 technology based on the traditional sgRNA vector is more suitable for knocking out a single coding gene because it targets only one site, and multiple sgRNA vectors/viruses are required for multiple coding genes and non-coding genes. The difficulty of operation is not small.
Legend: Single sgRNA system for editing at a single site
To address the need for simultaneous knockout and lncRNA knockout of multiple coding genes, Multiplex sgRNA technology emerged.
The Multiplex sgRNA system inserts different sgRNAs simultaneously, helping to address the need to simultaneously knock out multi-gene or non-coding gene editing. It is possible to insert 2~5 sgRNAs at the same time to meet different experimental needs.
The knockout strategy for multiple encoded genes can be:
Legend: Designing two or more specific sgRNA sequences encoding genes, inserting vectors to knock out multiple coding genes after one operation
The strategy for non-coding genes or regions can be:
Caption: Design a specific sgRNA sequence for both non-coding genes or regions to be knocked out, and target the entire non-coding region to achieve non-coding gene knockout.
For pathway study students, it is sometimes necessary to suppress multiple pathways at the same time, and pathway inhibitors are still very expensive. If you have reliable sgRNA sequences for key genes, you can use CRISPR/CAS9 technology like pathway inhibitors. To suppress multiple signal paths at the same time, greatly improving the cost performance. For the students of the Car-T study, when editing the T cell to make UCAR-T, it is necessary to simultaneously knock out multiple checkpoint genes to standardize and mass-produce the CAR-T cells. At this time, it is convenient to introduce multiple sgRNAs at the same time. Meet this requirement.
There are other gospels that are suffering from special high-experience requirements. The Jikai gene has launched the Multiplex sgRNA system , which inserts up to 5 sgRNA targets simultaneously with up to 5 genes and 2 lncRNAs. Knockout work, is it awesome!
Long press and pay attention
Cosmetics are a mixture of various raw materials processed by reasonable allocation. There are many kinds of cosmetics with different properties. According to the cosmetic raw material performance and use, can be divided into two categories of substrate raw materials and auxiliary raw materials. The former is a kind of main raw material of cosmetics, which occupies a large proportion in cosmetics formula and plays a major role in cosmetics. The latter play a role in shaping, stabilizing, or imbuing cosmetics with color, fragrance and other properties that are extremely important in small amounts in cosmetic formulations. Cosmetics is with natural, synthetic or extract all sorts of action different material as raw material, via heating, agitation and emulsification wait for production program processing and become chemical mixture material.
tryptamine labs review,tryptamine supplement,tryptamine high
Shaanxi YXchuang Biotechnology Co., Ltd , https://www.peptide-nootropic.com