Leaf lettuce cultivation technology

The leaf is a vegetable that is warm and fertile, and its suitable growth temperature is 15--25 ° C. It can be planted in all four seasons, with November to May the following year as the most suitable planting period.

I. Land preparation, seeding and seedling raising

1. Land preparation: The selection of seedlings requires sandy soil with loose soil, good air permeability, and water and fertilizer retention. After the seedlings are bleached with loose soil, they shall be hoisted according to 1.2-1.5 meters of ditch, and the loquat shall be 25 cm high. Seedlings should be fully basal fertilizers, mainly rotten organic fertilizers. The basal fertilizers and soil can be fully mixed before seeding.

2. Sowing: It is required to choose fresh and full-colored seeds, and do a good job of pre-seeding treatment. The seeds are sunning and germinated. The amount of seeds per mu is 0.1-0.12 kg.

3. Seedling cultivation: After sowing, cover the seeds with a thickness of about 1 cm and drench with water to keep them moist. The seedlings are unearthed 3 to 10 days after sowing. When it grows to 2--3 true leaves, thinning should be carried out. Thinning should be carried out in sunny and warm weather, and watering and seedlings should be drenched immediately after thinning.

Second, transplant seedlings. Fields for planting lettuce for leaves require the selection of convenient drainage and irrigation, and sandy loam soil with a neutral or slightly acidic pH. The field is prepared by raking the ground after applying basic fertilizer. The width of the hoe is 1.5 meters (including ditch), and it is planted horizontally after hoeing. The size is 10 × 13 cm. When the seedlings grow to 4--5 true leaves, they can be transplanted and planted , Planting 3500--4000 plants per acre. After transplantation, drench the foot with root water.

Third, fertilizer and water management. After the vegetable seedlings are planted, it is necessary to grasp the fertilizer and water management in a timely manner. The seedlings after planting should be watered every day for 3 to 4 days on a sunny day, to keep the moist surface moist, and to eliminate field water on rainy days. 10 to 12 days after planting, fertilizer can be topdressed. Difficult and thin application is required, and rotten manure water is used as a fertilizer. Compound fertilizer can also be applied. Do not apply chemical fertilizer 10 days before harvesting to avoid degrading quality.

Fourth, timely harvest. The leaf lettuce should be harvested in a timely manner. When it grows to 10-12 leaves, it is the most suitable harvest period. At this time, the quality is high.

Fifth, pest control. The diseases and insect pests that occur on leaf lettuce are: powdery mildew, blight, ulcer, aphid, cabbage worm and so on. Disease prevention and treatment drugs include: carbendazim, mirex, tobbutin, etc. Pest control drugs include trichlorfon and some pyrethroid pesticides.

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PCR Reagents

PCR Reagents

Real time PCR Kit for Monkeypox Virus is used for in vitro qualitative nucleic acid detection of Monkeypox Virus in Human serum, lesion exudate samples and scab specimens. Primer sets and FAM labeled probes are designed for specific detection of Monkeypox Virus, Human RNase P gene extracted concurrently with the test sample provides an internal control to validate nucleic extraction procedure and reagent integrity. Probe targeting human RNase P gene is labeled with VIC.


Intended Use
The test is intended for in vitro qualitative detection of monkeypox virus
(MPXV) antigen in human oropharyngeal swab, whole blood or skin lesion
materials, including lesion exudate, lesion roofs, lesion crusts.
The test is used for monkeypox virus antigen test of monkeypox suspected
populations. Positive result of the antigen test can be used for early triage and
rapid management of suspected populations, but it cannot be used as
diagnosis basis of monkeypox infection. Negative results do not rule out
monkeypox infection and should not be used as the sole basis for treatment or
patient management decisions. Further nucleic acid detection should be
carried out for suspected population whose antigen test result is positive or
negative.
The test is only for professional use, not suitable for family test. The test result
is only for clinical reference and it is recommended to conduct comprehensive
analysis of the disease condition in combination with clinical manifestations of
patients and other laboratory tests.
Test Principle
According to the gold immunochromatographic test principle, the
nitrocellulose membrane is coated with MPXV mAb 2 and goat anti-mouse IgG
antibody, the gold conjugate pad is labeled with MPXV mAb 1. When the
antigen is contained in the sample, the antigen binds with the corresponding
gold labeled monoclonal antibody to form a compound, moving forward under
the chromatography, then combines with the coated antibody in the test line
to form Au-MPXV mAb 1-antigen-MPXV mAb 2 complex to condense into a red
band (Test line, T), indicating a positive result. When the sample does not
contain antigen, complex cannot be formed in the test line, and no red band
appears, indicating a negative result.
No matter whether the sample contains antigen or not, the gold labeled
monoclonal antibody will combine with the coated goat anti-mouse IgG
antibody at the control line to form a Au-MPXV mAb 1-goat anti-mouse IgG
antibody complex and condense into a red band (Control line,C)


Pcr Reagents,Reagents Used In Pcr,Reagents Required For Pcr,Pcr Reagent Kit

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