1 Materials and Methods
1.1 The material was used as the material of the old-leaved rubber tree twigs of the current year. After conventional surface disinfection, single buds or double bud stem segments were cut into the medium. One month later, when the test tube plants were overgrown with the culture bottles, subculture was performed. .
1.2 Methods MS + NAA (naphthaleneacetic acid) 0.05mg/L + white sugar 2.0% + agar 0.36% as the basic medium, adding 0.1, 0.5, 1.0mg/L 6-BA (6-benzylamino adenine) And with MS+6-BA 0.1mg/L+IAA (indole-3-acetic acid) 0.05mg/L+white sugar 2.0%+agar 0.36% as the basic medium, add 1.0, 5.0, 10.0 mg/L of hydrolyzed milk Protein (LH), observe rubber tree growth. Using 1/2MS + white sugar 2.0% + agar 0.36% as basic medium, 0.1, 0.2 mg/L of IBA (Indole-3-butyric acid) (A1, A2), NAA (B1, B2), IAA were added. (C1, C2) Observe the growth and rooting of the rubber tree, and select the best growth and rooting medium. All media had a pH of 5.8.
1.3 Transplanting Method of Tube Seedlings Rooted rubber tree test tube seedlings, in the cultivation room, remove the sealing film from the culture flask, exercise for 1 to 2 days in the room, and then remove the test tube seedlings from the culture flask and wash the root culture medium; In a plastic arch shed with 10cm thick perlite on the ground, a shade net is used to cover the light outside the arch shed, the temperature inside the shed is maintained at 20°C to 30°C, and the water is sprayed once every 2 to 3 days in sunny days, and the arch shed is ventilated after 25 to 30 days. Transplant into pots.
2 Results and Analysis
2.1 The effect of 6-BA on the growth of rubber tree test-tube seedlings The test results showed that in the MS+NAA 0.05mg/L + white sugar 2.0% + agar 0.36% medium, add 6-BA 0.1 ~ 1.0mg/L, with With the increase of 6-BA concentration, the proliferation rate of rubber tree decreased, the callus at the base of stem segment was more, and the young shoots and leaves became smaller.
2.2 Effect of LH on the growth of rubber tree plantlets The results showed that 1.0 to 10.0 mg/L of LH was added to MS+6-BA 0.1 mg/L+sugalt 2.0%+agar 0.36% medium, only 1.0 mg/L. The LH of L can increase the multiple times of the proliferation of rubber tree seedlings, and the LH of 5.0-10.0 mg/L is unfavorable to the proliferation of test tube seedlings.
2.3 The effects of auxin on the proliferation and rooting of rubber tree test-tube seedlings showed that adding 0.1 to 0.2 mg/L IBA, NAA and IAA in the medium of 1/2MS + 2.0% of white sugar + 0.36% of agar can promote rubber. The effects of the growth and rooting of the test-tube seedlings in the tree and the combined use of IBA, NAA and IAA were better than those of the three alone, indicating that these three auxins have interactions in the rubber tree tissue culture. Therefore, the best medium for rubber tree test tube propagation is 1/2MS + IBA 0.2mg/L + NAA 0.2mg/L + IAA 0.2mg/L + white sugar 2.0% + agar 0.36%. In this medium, the rubber The test tube seedlings of the saplings achieved growth and rooting in one step. The monthly multiplication rate was 5.56, the rooting rate was 100%, and 8.4 roots per plant.
2.4 Rubber tree test tube seedling transplanting technology Rooting rubber tree seedlings The survival rate of transplanting test tube seedlings into plastic arch sheds was 75.9% (645/850), and the survival rate of transplanting into pots was 93.3% (602/645). The transplanting survival rate was 70.8% (602/850). After 6 months the height of the plants was 30 to 46 cm with 9 to 15 leaves.
3 Conclusion
(1) When 6-BA was added to the medium, the proliferation times of the test-tube seedlings of the big-leaved rubber tree decreased, the number of callus increased, and the number of new shoots and leaves became smaller. (2) LH1.0mg/L can increase the proliferation of the big leaf rubber tree plantlets, while the 5.0 ~ 10.0mg/L LH is unfavorable to the proliferation of the plantlets. (3) The best medium for rubber tree test tube propagation is 1/2MS + IBA 0.2 mg/L + NAA 0.2 mg/L + IAA 0.2 mg/L + white sugar 2.0% + agar 0.36%. In this medium, the rubber Tree plantlet proliferation and growth and rooting is completed in one step.
Sprinkler irrigation and micro-irrigation automatic control equipment With the development of economy, water resources, energy shortage and labor cost increase, more and more water-saving irrigation systems will adopt automatic control. This article focuses on the advantages and classification of automated irrigation.
The advantages are as follows:
(1) It is possible to truly control the amount of irrigation, irrigation time and irrigation cycle in a timely and appropriate manner, thereby increasing crop yield and significantly improving water utilization.
(2) Saving labor and operating expenses.
(3) The work plan can be arranged conveniently and flexibly, and the management personnel do not have to go to the field at night or other inconvenient time.
(4) Since it can increase the effective working time every day, the initial capital investment in pipelines, pumping stations, etc. can be reduced accordingly.
classification:
First, fully automated irrigation system
The fully automated irrigation system does not require direct human involvement. The pre-programmed control procedures and certain parameters that reflect the water requirements of the crop can automatically open and close the pump for a long time and automatically irrigate in a certain order. The role of the person is simply to adjust the control program and overhaul the control equipment. In this system, in addition to emitters (heads, drip heads, etc.), pipes, fittings, pumps, and motors, it also includes central controllers, automatic valves, sensors (soil moisture sensors, temperature sensors, pressure sensors, water level sensors, and rain sensors). Etc.) and wires.
Second, semi-automatic irrigation system
In the semi-automated irrigation system, no sensors are installed in the field. The irrigation time, irrigation volume and irrigation period are controlled according to pre-programmed procedures, rather than feedback based on crop and soil moisture and meteorological conditions. The degree of automation of such systems is very different. For example, some pump stations implement automatic control, and some pump stations use manual control. Some central controllers are only one timer with simple programming function, and some systems have no central control. The controller, but only some of the sequential switching valves or volume valves are installed on each branch pipe.
Automated irrigation is the trend of the times. In the future water-saving irrigation projects, more and more automated irrigation systems will be applied.
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